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PPARγ-primed macrophage facilitated functional xenogeneic tooth root regeneration. Odontogenesis-related proteins were evaluated by immunohistochemistry (A) The regenerated pulp-dentin complex-like tissue were positive for dentin marker (DMP-1), odontoblast-related markers (DSPP, periostin), ECM components marker (Col-I), and neuronal marker <t>(β-tubulin</t> Ш), in line with those of Native. Merely expressed DSPP and periostin were observed in the control group. Scale bars, 100 μm. (B) The regenerated cementum-PDLs-bone complex-like tissue was positive for DSPP, specific PDLs marker (periostin), collagen fibrils marker (Col-I, Col-III), and neuronal marker (β-tubulin Ш), in line with those of Native. Merely expressed DSPP and periostin were observed in the control group. Scale bars, 100 μm.
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PPARγ-primed macrophage facilitated functional xenogeneic tooth root regeneration. Odontogenesis-related proteins were evaluated by immunohistochemistry (A) The regenerated pulp-dentin complex-like tissue were positive for dentin marker (DMP-1), odontoblast-related markers (DSPP, periostin), ECM components marker (Col-I), and neuronal marker <t>(β-tubulin</t> Ш), in line with those of Native. Merely expressed DSPP and periostin were observed in the control group. Scale bars, 100 μm. (B) The regenerated cementum-PDLs-bone complex-like tissue was positive for DSPP, specific PDLs marker (periostin), collagen fibrils marker (Col-I, Col-III), and neuronal marker (β-tubulin Ш), in line with those of Native. Merely expressed DSPP and periostin were observed in the control group. Scale bars, 100 μm.
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PPARγ-primed macrophage facilitated functional xenogeneic tooth root regeneration. Odontogenesis-related proteins were evaluated by immunohistochemistry (A) The regenerated pulp-dentin complex-like tissue were positive for dentin marker (DMP-1), odontoblast-related markers (DSPP, periostin), ECM components marker (Col-I), and neuronal marker (β-tubulin Ш), in line with those of Native. Merely expressed DSPP and periostin were observed in the control group. Scale bars, 100 μm. (B) The regenerated cementum-PDLs-bone complex-like tissue was positive for DSPP, specific PDLs marker (periostin), collagen fibrils marker (Col-I, Col-III), and neuronal marker (β-tubulin Ш), in line with those of Native. Merely expressed DSPP and periostin were observed in the control group. Scale bars, 100 μm.

Journal: Bioactive Materials

Article Title: Recruited CD68 + CD206 + macrophages orchestrate graft immune tolerance to prompt xenogeneic-dentin matrix-based tooth root regeneration

doi: 10.1016/j.bioactmat.2020.09.029

Figure Lengend Snippet: PPARγ-primed macrophage facilitated functional xenogeneic tooth root regeneration. Odontogenesis-related proteins were evaluated by immunohistochemistry (A) The regenerated pulp-dentin complex-like tissue were positive for dentin marker (DMP-1), odontoblast-related markers (DSPP, periostin), ECM components marker (Col-I), and neuronal marker (β-tubulin Ш), in line with those of Native. Merely expressed DSPP and periostin were observed in the control group. Scale bars, 100 μm. (B) The regenerated cementum-PDLs-bone complex-like tissue was positive for DSPP, specific PDLs marker (periostin), collagen fibrils marker (Col-I, Col-III), and neuronal marker (β-tubulin Ш), in line with those of Native. Merely expressed DSPP and periostin were observed in the control group. Scale bars, 100 μm.

Article Snippet: The following primary antibodies were used: mouse anti-monkey/human DMP-1 and βⅢ-tubulin (Millipore, Billerica, MA, USA); rabbit anti-monkey/human periostin, COL-3 (Abcam, Cambridge, MA, UK); mouse anti-monkey/human COL-1 (Abcam, Cambridge, MA, UK); and mouse anti-monkey DSPP (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Functional Assay, Immunohistochemistry, Marker